Strategies for HPLC Method Development
Tuesday 4th August 2020 at 15:00 BST/16:00 CEST/10:00 EDT
(Duration: approx. 40 min followed by up to 20 min for questions)
The benefits of using a strategic approach for developing HPLC methods are easily apparent. The numerous possible chromatographic parameters in a typical HPLC method make choosing the most suitable ones for a particular separation very daunting. In particular, how to select one column from the hundreds available? There are a number of different strategies which can be applied, these include: trial and error, changing one variable at a time; finding a method in the literature or finding a method in the literature for a similar compound; and sophisticated column screening experiments combined with computer modelling, peak tracking methods, experimental design and column comparison tools. In this presentation, current recommended method development strategies are reviewed and compared to give attendees an appreciation of the types of strategies which may be applied, so that they can identify the one which is most applicable for their method development needs.